For LC-MS analysis, the samples were re-dissolved in 100 μL acetonitrile/water (1:1 ... was divided into new centrifuge tubes in equal parts (400 μL per tube) and frozen at −80°C. For use, the ...

Briefly, frozen-thawed semen was diluted to 1.2 × 10 7 cells/mL ... The lipid extracts were re-diluted in 200 μL of a 90/10 mixture of isopropanol and acetonitrile and centrifuged at 14,000 g for 15 ...

The samples were frozen in liquid nitrogen and stored at −80°C until ... water (1:1 [v/v]) and methanol:acetonitrile (2:8 [v/v]) by homogenization and centrifugation. Six internal standards ...

Here's what you can do if your pipes are frozen, and how to prevent it from happening again if your temperatures will drop below freezing again Wednesday night. The American Red Cross offered ...

If you turn on a faucet and no water comes out, or only a trickle, it could indicate a frozen pipe. Discolored water: If you do get water, but it's discolored (brown, reddish, or cloudy), it could ...

In 2020-2021, a mysterious skin disease affected fishermen in Senegal. High levels of Portimine A toxin, produced by the ...

Frozen vegetables are a convenient way to consume more produce, but a new study suggests that many people are not using them safely. For the study, researchers analyzed survey data from more than ...

In their frozen state, the alligators take about one breath every minute, he explained. "Their heart rate is down to about three beats per minute," Saurage added. "That animal is suspended in ...

Dramatic video shows a New Jersey cop jumping into frigid waters to save an 11-year-old boy who fell through ice into the middle of a frozen pond. The unidentified boy wandered about 75 yards ...