Expression of the cloned cDNA in insect and mammalian cells yielded 65-kDa and 50-kDa recombinant heparanase proteins.

Critical Step The bicistronic expression vector pIRES can be used to generate stable cell lines expressing GFP-tagged proteins.

Antibody–siRNA conjugates (ARCs) are a type of promising drug modalities for cancer therapy. However, initial reports of ARCs present the gene knockdown effect only in limited tissues (e.g., muscles) ...

Bioluminescent imaging is helping researchers better understand the effectiveness of tissue regeneration enhancers delivered to injured heart tissue by different adeno-associated virus vectors.

The mRNA metabolism passively shapes the levels of an mRNA modification called m6A within a steady-state cell and upon stress.