As RNA-Seq is quantitative, it can be used to determine RNA expression levels more accurately than microarrays. In principle, it is possible to determine the absolute quantity of every molecule in ...
Principle of Nanopore Sequencing: DNA/RNA is passed through protein nanopores embedded in a non-conductive polymer membrane with the assistance of helicase, while a voltage is applied across the ...
"As a proof of principle, we sequenced aged frontal cortex ... the RNA patterns in complex diseases and deep long-read RNA sequencing will be a necessary tool for that work.