Tumor necrosis factor-α (TNF-α) mRNA served as the target gene and ... Thus, the overall efficiency of RT– and real-time PCR in detecting the housekeeping gene in a limited number of picked ...

This does not yet offer an increase in accuracy compared to well-optimized competitive techniques using endpoint PCR product detection. Another approach for quantitation of mRNA levels by RT-PCR ...

or real-time RT–PCR. Fast and accurate, real-time RT–PCR has been critical in detecting, tracking and studying COVID-19. But what is it, and how does it work? Let us explain.

RT-PCR test quantifying CP mRNA in blood can detect hepatocellular carcinoma early—especially in AFP-negative cases—offering ...

The technology involves three steps. First, reverse transcriptase makes DNA copies of the mRNA, using a chimeric DNA-RNA oligo-dT primer. Next, DNA polymerase copies the DNA, producing a ...

Real-time PCR was employed to determine whether miR-377 could reduce the levels of MYC mRNA in transfected PCa cell lines (PC-3 and DU145) and change in the mRNA levels of BCL-2/Bax, PTEN ...

burgdorferi. Using quantitative PCR to detect the bacteria, the researchers found that 80 percent of mice vaccinated with OspA mRNA were protected from B. burgdorferi infection compared to 50 percent ...

Gen-Probe is currently developing the APTIMA HPV Assay, targeting E6/E7 mRNA from 14 carcinogenic ... including molecular beacons for real-time detection. (A) Two specific primers and three ...

These RNA molecules can interact with mRNA, influencing its stability ... RNA-based diagnostic tools, such as RT-PCR and RNA sequencing, are widely used for detecting and quantifying RNA in biological ...