In vitro kinase assay. PMNs were isolated, stimulated for 5 mins with IL-8 and immunoprecipitation done as described above.

A breakthrough assay is advancing how researchers observe gene expression in real time. Named QRIVTA (quantitative real-time ...

Thus, G-protein-coupled receptors can regulate NMDA receptor function indirectly through a PKC-dependent activation of the non-receptor tyrosine kinase (Src) signaling cascade.

During early drug development and preclinical development, researchers use in vitro cultured lymphocytes to induce cytokine ...

Antibody–siRNA conjugates (ARCs) are a type of promising drug modalities for cancer therapy. However, initial reports of ARCs present the gene knockdown effect only in limited tissues (e.g., muscles) ...

An experimental blood test can detect celiac disease, even when no gluten has been consumed, which may make the path to accurate diagnosis quicker and less painful.

Tyrosine residues in the activation loop and the conserved ATP-interacting Lys44 are also marked. (C) In vitro kinase assay showing autophosphorylation of wild-type full-length IKK2 (FL IKK2WT) upon ...

Notably, in vitro tyrosine kinase profiling and cellular activity-based protein profiling (ABPP) cooperatively indicated a specific inhibition of c-Src by (R)-LW-Srci-8. Intriguingly, (R)-LW-Srci-8 ...