In the work, the researchers added the vitamin biotin to those short DNA strands to turn the system into a sensor for the protein streptavidin. Then they added a DNA aptamer, a DNA strand that can ...
Shah and colleagues take advantage of the presence of maternal and somatic ribosomes in zebrafish and confirm their differential expression during development. The authors convincingly show that ...
The team demonstrated that the lilypad could be adapted to sense proteins by modifying the short DNA strands with biotin, enabling detection of streptavidin. Additionally, they incorporated a DNA ...
Primary antibodies were then incubated overnight at 4°C, succeeded by incubation with streptavidin-HRP or lexa Fluor secondary antibody for 2 h. Subsequently, the tissues were stained with ...
The primary antibody (Table S3) was incubated overnight at 4 °C. The following day, biotinylated secondary antibody (Reagent 3) and streptavidin‐horseradish peroxidase conjugate (Reagent 4) were ...
In standard procedures, binding is detected with Alexa Fluor 647-labeled streptavidin. The microarray facility will provide appropriate ‘anti-tag’ antibodies, as well as commercially available ...
Jung J, Sung JS, Kwon S, Bae HE, Kang M-J, Jose J, Lee M, Pyun J-C (2025) Transmembrane protease serine 2 (TMPRSS2) inhibitors screened from an Fv-antibody library for preventing SARS-CoV-2 infection.
Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. m 6 A RNA modification has been linked to ...
Sixth People’s Hospital, School of Medicine & School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, P. R. China Centre for Personalized Nanomedicine, Australian Institute ...