resulted in full-length cDNA sequences for 23 novel germ-cell–specific genes (Table 2). Figure 1: Expression of 23 novel germ-cell-specific genes in mouse tissues assayed by RT–PCR.

cDNA produced by a MessageBOOSTER cDNA Synthesis from Cell Lysates kit reaction eliminates the difficulties frequently encountered in qRT-PCR gene expression studies with very small numbers of cells.

GBU's reagent collection contains a library of open reading frames (ORFeome collaboration) consisting of about 18 600 fully verified cDNA clones ... in Gateway-compatible vectors but we can design PCR ...

Because uMRT provides full 5'-3' RNA transcript coverage, PCR amplicon location along a first-strand cDNA molecule synthesized by uMRT does not affect qPCR quantification. This greatly reduces the ...