This handbook explores key strategies for optimizing qPCR workflows, from sample preparation to data analysis, helping you ...

The findings, published on February 15, 2025, in Frontiers of Computer Science, address a key challenge in quantitative PCR (qPCR) primer design. Unlike existing software, which requires manually ...

Human extracellular matrices can be edited in their composition using the CRISPR/Cas9 system, leading to materials exhibiting tailored regenerative capacities.

Run the Primer Blast, we can select a primer set. This primer set spans an exon-exon junction in the coding sequence. Its potential unwanted products are either >1000 bp or having >= 4 mismatches on ...

primer design for double cortin like kinase 1 (Dclk1) for mouse mm10 reference that targets both short isoform exon 2 and long isoform exon 6 (NOT junction spanning) Navigate to Dclk1 gene for mm10 ...

Then ELISA, immunofluorescence staining, immunohistochemistry staining, and RT-qPCR were performed to detect the expressions ... and analyzed using the 2 −∆∆Ct method for relative quantification. The ...

A new discovery has reshaped our understanding of PCR chip technology: water vapor, not air expansion, is the primary culprit ...

We may earn an affiliate commission from links. If you’re seeking to achieve an airbrushed complexion, the best pore minimizing primers can do the trick, smoothing things over and helping you ...

DARPA chose Diraq and Silicon Quantum Computing for the Quantum Benchmarking Initiative, which will assess whether ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...