University of Wisconsin–Madison researchers targeting a group of hereditary neurodegenerative diseases have found success ...

Researchers have discovered that a protein complex that helps fight cancer cells also slows the growth of tuberculosis—a ...

we first investigated the impact of functional driver gene knockout on cancer cell viability in the DLBCL cell line OCI-LY3 in a non-mutation-specific manner. To ensure the reliability and efficacy of ...

Mice constitute the most widely used model since their retinal structure is similar to humans and they can easily provide knock-out/-down models ... Comparison of CRISPR/Cas9 nucleases, base editors, ...

It allows the evaluation of the quality of gene editing experiments using targeted next generation sequencing (NGS) data (`targeted`) as well as the discovery of important genes from knock-out or ...

Similarly, RbFox2 expression was increased in CRISPR-Cas9 generated CUL3-deficient HEK293 cells, in aorta isolated from SMC-specific CUL3-deficient mice, and in A7r5 cells expressing an siRNA ...

and all these fragments overlap only marginally with the mouse region. The CRISPR/Cas9 experiment to generate our knockout line might have generated potential off-target mutations. In order to rule ...

Ultimately, this could help with finding better ways to fight this deadly disease. Here we describe a CRISPR/Cas9-mediated gene knockout functional screening method in which lentiviral genomes ...