1Children’s Research Institute and the Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas. 10Howard Hughes Medical Institute, UT Southwestern Medical Center, ...

The 20 μl of pre-washed Dynabeads Protein G/A 1:1 mixture beads (Invitrogen) were added for binding and incubated at 4°C for 3 h. After washed with CL-IP wash buffer for 3 times, beads were collected ...

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Xi’an 710119, P. R. China Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Xi’an 710119, P.

This study reports valuable findings on the role of Layilin in the motility and suppressive capacity of clonal expanded regulatory T cells (Tregs) in the skin. Although the strength of the study is ...

siRNA transfection experiments were performed with Lipofectamine 3,000 Reagent (Invitrogen L3000-001 ... Then, antibodies were conjugated to 30 µL protein A/G magnetic beads (Pierce, 26,262) to ...

We cultured zebrafish liver (ZFL) cells (originally obtained from the American Type Culture Collection) in 50% L-15 (Invitrogen, Carlsbad, CA, USA), 35% DMEM-HG (Invitrogen), and 15% Ham's F12 medium ...