MSK researchers shed new light on G-quadruplexes, a type of secondary DNA structure that has attracted attention as a potential therapeutic target in cancer.

Determining the number of copies of a double stranded DNA template (be it genomic DNA, plasmid or an amplified fragment) is essential for many genetic quantification applications in research and ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

Once transcription is initiated, the DNA double helix unwinds and RNA polymerase reads the template strand, adding nucleotides to the 3′ end of the growing chain (Figure 2b). At a temperature of ...

Kokoris says that after years of optimization, the enzyme Roche researchers are currently working with yields sequencing reads that are 99.35% faithful to the original DNA template sequence.

Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...

And one of the things it can get stuck on is the DNA template itself, which can adopt alternative structures in certain contexts. For example, regions of the genome that are rich in guanine bases ...