Frontiers1mon
Deconvolution of the Response to Bacillus Calmette–Guérin Reveals NF-κB-Induced Cytokines As Autocrine Mediators of Innate Immunity
After 2–3 weeks of growth, an aliquot of the culture was harvested and washed in 1× Dulbecco’s phosphate-buffered saline (DPBS; Gibco, France) for 8 min at 4,000 rpm. The bacterial pellet was ...
Frontiers2y
Long-term effects of early postnatal stress on Sertoli cells
Cells obtained after enzymatic digestion of testis were resuspended in 5–10 ml FACS buffer (1x DPBS [Gibco] supplemented with 1% Pen-Strep, 1% FBS, 10 mm HEPES, 1 mm pyruvate [Gibco] and 1 mg/ml ...