Isolation of total RNA Total RNA was extracted ... SMART II A oligonucleotide provided in the kit. Two 5'-gene-specific primers, svchs5-1 and svhcs5-2 (Table S1), were designed for 5'-RACE. The first ...

Patients in cohorts 1 and 3 were stratified according to cirrhosis status. HCV RNA levels were determined using the Ampliprep/TaqMan HCV Test, V.2.0 (Roche Molecular Systems, Branchburg, New Jersey, ...