Isolation of total RNA Total RNA was extracted ... SMART II A oligonucleotide provided in the kit. Two 5'-gene-specific primers, svchs5-1 and svhcs5-2 (Table S1), were designed for 5'-RACE. The first ...

Patients in cohorts 1 and 3 were stratified according to cirrhosis status. HCV RNA levels were determined using the Ampliprep/TaqMan HCV Test, V.2.0 (Roche Molecular Systems, Branchburg, New Jersey, ...

Extraction of viral RNA - HCV RNA was extracted from serum samples using a QIAamp Viral RNA kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. Nucleotide sequencing - The ...

High Precision with UMIs and Optimized Indexing The miRVEL Discovery incorporates Unique Molecular Identifiers (UMIs), eliminating PCR duplicates ... Small RNA-Seq Library Prep Kit features ...

HIV–HCV coinfection is associated with a higher HCV RNA load and rapid disease progression to cirrhosis compared with HCV-monoinfected patients. HCV clearance is associated with vigorous and ...

The hepatitis C virus (HCV) is a positive sense RNA virus that causes chronic liver disease in roughly 2% of the world’s population. HCV causes profound morbidity and mortality and is a leading cause ...