The mRNA targets of miRNA can be determined by using bioinformatics methods and bioassays for monitoring the amounts of target mRNA. In plants, miRNA binding sites are usually found in the coding ...

Since a miRNA may regulate many of its targets at the translational level without affecting mRNA abundance, proteomic methods are best suited for revealing the full spectrum of miRNA targets.

causing translational repression and/or mRNA degradation 1. Despite recent advances in large-scale target screening techniques, experimental validation of miRNA targets remains cumbersome ...

"It is striking that in both mice and humans, the same miRNA binds in two places on the SRL mRNA,", said Marzluff, ...