Restriction sites, like airline hubs, aren't always where you need them, and sometimes they aren't there at all. Recombinase-based methods allow scientists to move pieces of DNA from plasmid to ...

Once pure samples of DNA have been prepared, it can be manipulated in several ways; cut at specific sites and then ... you will carry out restriction digestion of the genomic clone, in order to cut ...

Plasmid vectors pET11a-link-NGFP and pMRBAD ... Alternatively, the loss of the unique linker restriction sites can also be used as a diagnostic. Confirm that the GFP fusions are the correct ...

The Ti plasmid is removed from the agrobacterium cell, and a restriction enzyme cleaves the T-DNA at a specific restriction site. Subsequently, foreign DNA, which has been cleaved by the same enzyme, ...