Reversed-phase column (Brownlee RP-300 cartridge; 100 mm length × 2.1 mm inner diameter, octylsilica 300 Å pore size, 7 μm diameter of the particle; PerkinElmer) Size-exclusion columns ...

Cell or tissue lysates are arrayed on nitrocellulose-coated slides (Grace Bio-labs, Bend, OR) in triplicate using the Aushon 2470 Arrayer (Aushon BioSystems, Billerica, MA) (technical replicates).

such as immunohistochemistry and reverse-phase protein microarrays. Recent advances in performing matrix-assisted laser desorption/ionization imaging mass spectrometry on FFPE tissue makes high ...

Our instrument capabilities have increased over the years as well. Beyond the traditional ion-exchange liquid chromatograph, we also use a reverse-phase liquid chromatograph that has up to 100 times ...

ProteomeLab PF2D (Beckman Coulter) is a high resolution 2-dimensional liquid protein fractionation system using chromatofocusing in the first dimension and a high capacity reverse phase HPLC column in ...