Nature12d
A novel role for CCL3 (MIP-1α) in myeloma-induced bone disease via osteocalcin downregulation and inhibition of osteoblast function
CCL3 and osteopontin (OPN) expression was analyzed by ELISA assays purchased from R ... and signal detected with the Streptavidin-horseradish peroxidase system provided by the TSA kit (Perkin ...
Frontiers25d
Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysis
Streptavidin (100 μg/mL), radioimmunoprecipitation assay buffer, Bradford protein assay kit, and enzyme-linked immunosorbent assay (ELISA) kit were obtained from Beijing Solarbio Company. TB Green ...
BMJ10d
Identification and characterisation of citrullinated antigen-specific B cells in peripheral blood of patients with rheumatoid arthritis
Methods Differentially labelled streptavidin and extravidin tetramers were conjugated ... for the presence of antibodies against citrullinated protein antigens (ACPA) by ELISA. Cells were ...
Frontiers12d
A human monoclonal antibody isolated from Japanese encephalitis virus vaccine-vaccinated volunteer neutralizing various flaviviruses
In brief, ELISA plate was coated with JEV viral particles (4 × 10 ... using a cocktail of biotinylated antibodies against other cells except B cells plus streptavidin-conjugated microbeads (Miltenyi, ...
BMJ20d
Antibodies and B cells recognising citrullinated proteins display a broad cross-reactivity towards other post-translational modifications
Objective Autoantibodies against antigens carrying distinct post-translational modifications (PTMs), such as citrulline, homocitrulline or acetyllysine, are hallmarks of rheumatoid arthritis (RA). The ...
C&EN24d
Innovative Ricin Toxin Detection: Unraveling Apurinic/Apyrimidinic Lyase Activity and Developing Fluorescence Sensors
Streptavidin (SA) at 5 mg/mL was immobilized on the test line of the sensor strip, and Green-fluo microspheres (10× dilution of stock solution) were immobilized on the control line. The reaction mix ...
eLife19d
C-C chemokine receptor 4 deficiency exacerbates early atherosclerosis in mice
This valuable study provides in-vivo evidence that CCR4 regulates the early inflammatory response during atherosclerotic plaque formation. The authors propose that altered T-cell response plays a role ...
gut.bmj6d
BMP-9 interferes with liver regeneration and promotes liver fibrosis
4 Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology and University of Tuebingen, Stuttgart, Germany 5 German Red Cross Blood Service Baden-Württemberg-Hessen and Institute for Transfusion ...