A new discovery has reshaped our understanding of PCR chip technology: water vapor, not air expansion, is the primary culprit ...

Q: What should I do if my primer Tm value is too low or too high? A: The program attempts to optimize primer design, but you can try adjusting the homology arm length or modifying the fragment order.

We may earn an affiliate commission from links. If you’re seeking to achieve an airbrushed complexion, the best pore minimizing primers can do the trick, smoothing things over and helping you ...

This handbook explores key strategies for optimizing qPCR workflows, from sample preparation to data analysis, helping you ...

A primer is applied after the final step of your skincare routine and just before your first base product. Much like an artist will prime their canvas before applying the paint, a primer will help ...

The findings, published on 15 Feb 2025 in Frontiers of Computer Science, address a critical challenge in quantitative PCR (qPCR) primer design. Unlike existing software that requires manual ...

The target DNAs were cloned by PCR with primer pairs ASFV VP72 F/R and PCV2 Cap F/R (Supplementary Table S1). CRISPR-DT online design website was used for crRNA prediction (24). crRNA F and PCV2 crRNA ...

2021). The extracted RNA was stored at −80°C. For primer design, the complete genomes of 48 human alphacoronaviruses available at the Virus Pathogen Database and Analysis Resource (ViPR, Pickett et al ...

The survey reports that for processor development, the ratio of verification to design engineers is 5:1. Imagine having to hire five times more verification resources than design and having to respin ...

Primer Awards provide seed funding to develop new relationships, ideas and lines of research, and the generation of pilot data. The award aims to encourage scientists at all career stages to engage ...