With 10x Genomics, you get better data, fewer errors, and the confidence to get it right the first time. With costs as low as $600 USD per sample, you get high-resolution results unmatched by other single cell options, notably with up to 95% usable reads, letting you detect more genes at a lower sequencing cost.

In this workshop, you will be introduced to the single cell gene expression analysis journey. Analysis begins at experimental design. Together we will explore a set of neutrophil data starting with the 10x Cloud Analysis platform for raw data processing.

Mar 11, 2024 · 10x researchers demonstrated the powerful analytical capabilities of Chromium GEM-X Single Cell Immune Profiling (5’) v3 for clinical samples by analyzing dissociated tumor cells from human renal cell carcinoma samples.

Question: Is it necessary to sequence the Gene Expression library in a Barcode Enabled Antigen Mapping (BEAM) experiment? Answer: Yes, a Gene Expression (GEX) library is required to analyze Barcode Enabled Antigen Capture (BEAM)/Antigen Capture data in Cell Ranger. The cellranger multi pipeline runs into an error when a GEX library is not provided.

When performing a multiplexing experiment, a stock of RHS probes may be pooled and split across multiple tubes prior to adding the LHS CRISPR barcoded probe. See example below for pooling probes for a 4-plex multiplexing experiment.

The following criteria can help you decide which assay best fits your experiment. Customers should select the 5’ CRISPR assay if they meet the following criteria: They already have a pre-built, compatible guide RNA library they want to use in the single cell CRISPR screening workflow.

Chromium Single Cell CRISPR Screening assesses lentiviral guide RNA (sgRNA) transduced single cell input, providing a high-throughput and scalable approach to obtain gene expression profiles along with CRISPR-mediated perturbation phenotypes in the same single cell.

Dec 3, 2024 · This page is intended to familiarize Gene Expression Center clients with the many aspects of the various 10X Genomics single cell/single nuclei/spatial transcriptomics assays that are available through the University of Wisconsin-Madison Biotechnology Center.

Dec 11, 2019 · In this blog post, I will mix a mouse and a human 10x single cell RNAseq dataset in silicon and then map to the hybrid transriptome. We have the ground truth of which cell comes from which species so we can investigate the mapping rate.

Nov 6, 2024 · Using 10X Genomics’ technology CRISPR-mediated perturbations can be read out alongside a cell’s gene expression at single cell resolution. We offer both Single Cell 5′ CRISPR Screening (GEM-X and Next-GEM) and Single Cell 3′ CRISPR Screening (Next-GEM).