Single cell RNA sequencing (scRNA-seq) allows you to ask and answer questions that require single cell resolution on a scale that suits your experimental needs, from hundreds to millions of cells.

10x Genomics’ single cell RNA-seq (scRNA-seq) technology, the Chromium Single Cell 3’ solution, allows you to analyze transcriptomes on a cell-by-cell basis through the use of microfluidic partitioning to capture single cells and prepare barcoded, next-generation sequencing (NGS) cDNA libraries.

Mar 11, 2024 · Explore how Chromium GEM-X single cell RNA-sequencing can level up your research in developmental biology, cancer, neuroscience, drug discovery, & more.

10x Genomics Single Cell protocols require a suspension of viable single cells or nuclei as input. Minimizing the presence of cellular aggregates, dead cells, noncellular nucleic acids, and potential biochemical inhibitors of reverse transcription is critical to obtaining high-quality data.

1 day ago · Single-cell RNA sequencing CRISPR (perturb-seq) screens enable high-throughput investigation of the genome, allowing for characterization of thousands of genomic perturbations on gene expression. ... count using default parameters and mapping to the GRCh38-and-mm10-2020 reference genome from 10x Genomics. Using Seurat, cells with less than 15% ...

Approximately 40,000 cells were processed per lane of a 10X Genomics Single Cell 3′ HT with Feature Barcoding kit. ... T. Boenke, M. Senekowitsch, T. Krausgruber, D. Barreca, C. Bock, Ultra-high-throughput single-cell RNA sequencing and perturbation screening with combinatorial fluidic indexing. Nat. Methods 18, 635–642 (2021). Crossref ...

Jan 30, 2025 · Barcoded primer gel beads, oil-surfactant solution, and single cells with GemCode Single-Cell 3′ reagents are combined in 10X Genomics to form gel bead in emulsions, known as GEMs. Reverse transcription occurs within the GEMs, making their formation crucial for the success of scRNA-seq. Hydrogel beads are used in the 10X Genomics and inDrop ...

Single cell RNA-seq allows us to study cellular responses, identify rare cell types, explore cell states, and uncover novel biomarkers. It has revolutionized our understanding of cell biology and disease mechanisms. What are the key steps in a scRNA …

2 days ago · 10x Genomics offers a comprehensive suite of solutions tailored for various single-cell and spatial genomics applications. Each product is designed to address specific research objectives, utilizing unique mechanisms and providing specialized features. Below is an overview of these products, detailing their goals, mechanisms, key features, and associated analysis tools.

What are the best practices for flow sorting cells for 10x Genomics assays? Are there considerations for small cells or cells with low RNA? Are fresh frozen tissue samples compatible with Single Cell RNA sequencing? Can viral transcripts be detected with the Single Cell Assays? See all 32 articles