Aug 9, 2024 · This protocol outlines methods to obtain leukocytes, bone marrow mononuclear cells (BMMCs), and peripheral blood mononuclear cells (PBMCs) for use with 10x Genomics Single Cell RNA Sequencing protocols.

May 26, 2016 · Discover differentially expressed genes, visualize your favorite genes, and explore your data with our visualization software. Peripheral blood mononuclear cells (PBMCs) from a healthy donor (same donor as pbmc6k). PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

Users looking to isolate PBMCs can collect samples in these tubes and perform PBMC isolation using a ficoll gradient and SepMate tube ( follow option 3 of our demonstrated protocol Isolation of Leukocytes, Bone Marrow, and Peripheral Blood Mononuclear Cells …

Answer: Instructions for isolating PBMCs can be found in the Isolation of Leukocytes, Bone Marrow and Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing Demonstrated Protocol (CG000392). The following collection tubes and isolation methods have been validated for use with 10x Genomics single cell assays:

May 3, 2021 · Discover differentially expressed genes, visualize your favorite genes, and explore your data with our visualization software. Cryopreserved human peripheral blood mononuclear cells (PBMCs) from a healthy female donor aged 25 …

This protocol outlines cryopreservation and thawing of human peripheral blood mononuclear cells (PBMCs) for use with 10x Genomics Single Cell protocols. While this Demonstrated Protocol is specific to PBMCs, the protocol may be used as a basis for handling other primary cells in preparation for use in the 10x Genomics Single Cell protocols.

This protocol outlines methods to obtain leukocytes, bone marrow mononuclear cells (BMMCs), and peripheral blood mononuclear cells (PBMCs) for use with 10x Genomics Single Cell RNA Sequencing protocols.

Jul 6, 2023 · Here we use scRNA-seq to sequence more than 30,000 cells from human PBMC samples to high depth of coverage (>100,000 reads/cell). We provide data from resting and stimulated PBMCs. We used...

In this example, we will be analyzing a scRNA-seq dataset of 8K human peripheral blood mononuclear cells (PBMCs) freely available from 10X Genomics. The raw dataset can be downloaded from the 10X Genomics website. We will show you how to run through the whole MAESTRO pipeline from the raw sequencing fastq files to the final results.

May 29, 2019 · Discover differentially expressed genes, visualize your favorite genes, and explore your data with our visualization software. Peripheral blood mononuclear cells (PBMCs) from a healthy donor (the same cells were used to generate 5k_pbmc_v3_nextgem). PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).