What is multiplex PCR? - Front Line Genomics
Jul 26, 2021 · Multiplex PCR is a technique whereby PCR is used to amplify several different DNA sequences simultaneously. It is a type of target enrichment approach. It was first described in 1988 as a method to detect deletion mutations in the dystrophin gene – the largest known human gene. The dystrophin complex connects the cytoskeleton of muscle cells ...
Sample Preparation for NGS – A Comprehensive Guide
Aug 21, 2024 · Step #3: Amplification. This is an optional step, but it is usually required. It is dependent on the application of NGS and the sample size. Amplification becomes essential to obtain enough coverage for reliable sequencing for samples with small amounts of …
Long-read sequencing vs short-read sequencing
Jul 26, 2021 · Long-read sequencing. Long-read sequencing technologies are capable of reading longer lengths, between 5,000 and 30,000 base pairs. Therefore, they immediately address one of the main challenges faced by short-read sequencing. They sequence a single molecule, eliminating amplification bias, and generate a reasonable length to overlap a sequence ...
DNA Sequencing: How to Choose the Right Technology
Aug 6, 2021 · The global market for DNA sequencing is predicted to grow from $15.7 billion in 2021 to $37.7 billion by 2026. The rising prevalence of viral diseases, such as COVID-19, and the increasing cases of cancer globally are likely to drive genomic research and propel the industry at an even more rapid rate.
Everything you need (and want) to know about tardigrades
Oct 18, 2022 · Collect a clump of moss or lichen (dry or wet) and place in a shallow dish, such as a Petri dish. Soak in water (preferably rainwater or distilled water) for 3-24 hours. Remove and discard excess water from the dish. Shake or squeeze the moss/lichen clumps over another transparent dish to collect trapped water.
How-to: perform a genome-wide association study (GWAS)
Sep 10, 2024 · Genome-wide association studies are commonly used in the genomics world to identify variants associated with a trait or disease.During a GWAS, researchers will identify areas of the genome that show a strong signal linked to the trait in question; that is, in the discovery cohort, areas or specific markers in which variation is more common than in the controls.
DNA amplification techniques - Front Line Genomics
Aug 6, 2021 · DNA nanoball generation: Step #1: The DNA is fragmented and adapter sequences are attached. Step #2: A splint oligonucleotide hybridizes to the ends of the fragments, causing the DNA to form a circle. Step #3: The remaining linear DNA strands are removed, leaving only completed circular single-stranded DNA templates. Step #4: The circular DNA is replicated by phi29 DNA polymerase and the newly ...
Homepage - Front Line Genomics
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A longstanding mystery: How do identical twins arise?
Oct 1, 2021 · Scientists have discovered DNA methylation differences that are unique to identical twins, helping to unravel the exact molecular events that lead to division of a zygote.. Monozygotic twins, or identical twins, result from the fertilisation of a single egg with a single sperm.At some point during the very early pre-implantation stages of …